The Enzymatic Synthesis of Protein. Iii. the Effect of the Hydrogen Ion Concentration on Peptic Synthesis. by Hardolph

The Substrate in Peptic Synthesis of Protein

1. Experiments are described in which it was observed that the yield of protein that can be synthesized by pepsin from a given peptic digest is highest when the hydrolyzing action of the pepsin is stopped as soon as all the protein has disappeared from the solution; and that the longer the digest is permitted to contain active enzyme the more the yield diminishes. 2. Exposure of the digest to a...

The Enzymatic Synthesis of Protein. Iv. the Effect of Concentration on Peptic Synthesis. by Henry Borsook

In the enzymatic hydrolysis and synthesis of proteins in VWO, the important factor, the factor upon which the direction and the degree of the reaction are dependent, is not the relative concentration of water, but t,he concentration of mat,erial in solution. This conclusion, pointed out by Moore, the authors have discussed at length in a previous paper (I). As shown there, the molecular concent...

Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase

Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...

Optimization of Enzymatic Synthesis of Ampicillin Using Cross-Linked Aggregates of Penicillin G Acylase

Penicillin G acylase from E. coli TA1 was immobilized by Cross-Linked Enzyme Aggregates (CLEA), a new method for immobilization. This biocatalyst and commercial immobilized penicillin G acylase (PGA-450) were used to study the effect of pH, temperature and substrate concentration on the synthesis of ampicillin from phenyl glycine methyl ester (PGME) and 6-aminopenicillanic acid (6-APA). Compare...

The effect of hydrogen ion concentration on the anatomy of tobacco